Nimesulide Based Novel Glycolamide Esters: Their Design, Synthesis, and Pharmacological Evaluation

Table 2

In vitro cytotoxic activity of compound 4 against HCT-15 human colon cancer cell line.

Entry	Compounds	% of cell death at various concentrations^a					IC50 (µM)
		1 µM	2 µM	5 µM	10 µM	25 µM
1	4a	0.94	6.89	8.15	8.77	20.30	n.d.
2	4b	0.62	7.20	7.50	18.50	28.50	n.d.
3	4c	0.31	12.5	2.50	7.52	31.00	n.d.
4	4d	3.43	4.53	21.69	35.78	49.38	n.d.
5	4e	1.88	6.58	13.16	17.55	28.80	n.d.
6	4f	5.88	14.46	20.22	25.00	42.40	n.d.
7	4g	6.00	7.96	14.46	18.62	41.91	n.d.
8	4h	0.31	7.52	8.77	12.20	19.40	n.d.
9	4i	7.35	12.37	14.95	16.29	48.89	n.d.
10	4j	6.74	9.43	25.61	35.90	51.22	24.40 ± 0.14
11	4k	0	5.96	12.20	30.70	55.79	22.40 ± 1.31
12	4l	21.69	21.93	24.50	30.02	66.05	18.92 ± 0.09

aAll the values are the average of the experiments done in triplicates. Etoposide (IC₅₀ = 9.85 µM) was used as a reference compound. n.d.: not done.