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Journal of Chemistry
Volume 2013, Article ID 819460, 6 pages
Research Article

A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples

Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd 8916871967, Iran

Received 31 May 2013; Accepted 31 July 2013

Academic Editor: Pranav S. Shrivastav

Copyright © 2013 M. Reza Shishehbore et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A sensitive and accurate method for quantitative determination of dopamine was introduced. The proposed method is based on inhibitory effect of dopamine on the oxidation of thionine by bromate in acidic media. The change in absorbance was followed spectrophotometrically at 601 nm. The dependence of sensitivity on the reaction variables was investigated and optimized to obtain the maximum sensitivity. Under optimum experimental conditions, calibration curve was linear over the range 0.2–103.3 μg mL−1 of dopamine. The relative standard deviations ( ) of 0.5, 1.0, 5.0, and 30.0 μg mL−1 of dopamine were 1.13, 1.02, 0.99, and 0.97%, respectively. The limit of detection was 0.057 μg mL−1 of dopamine. The effect of diverse species was also investigated. The developed method was successfully applied for the determination of dopamine in pharmaceutical and biological samples.