A Novel and Sensitive Method for the Determination of Vitamin (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction
Figure 3
Effect of concentration of PEG 10000 on the extraction efficiency. Experimental conditions: volume of buffer: 4 mL, pH of sample: 6.4, concentration of sodium sulfate: 20% w/w, temperature: 30°C, centrifuging time: 15 min, 100 ng/mL of B2.