A Novel and Sensitive Method for the Determination of Vitamin B_𝟐 (Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction

Table 3

Comparison of different methods for the determination of vitamin B₂ with aqueous two-phase extraction method.

Method	LOD	LR	RSD %	Recovery %	𝑅^2	Reference
MLC	3 ng/mL	0.5–25 𝜇g/mL	<3.3	—	0.99991	[16]
Acid hydrolysis	0.003 𝜇g/mL	0.02–1 𝜇g/mL	1.6	70.3	0.9999	[17]
Flow injection	0.005 𝜇g/mL	0.01–10 𝜇g/mL	—	>97.5	0.9986	[8]
HPLC	188 𝜇g/mL	—	2.0	>95	0.9992	[18]
Liquid chromatography	40 ng/mL	2–16 𝜇g/mL	<9	>87.5	0.9985	[4]
ATPE	0.2 ng/mL	1.3–320 ng/mL	3.6	>94.8	0.9991	This work