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Journal of Chemistry
Volume 2014, Article ID 237359, 5 pages
Research Article

Evaluation of Antibacterial Enrofloxacin in Eggs by Matrix Solid Phase Dispersion-Flow Injection Chemiluminescence

1Hebei Key Lab of Laboratory Animal Department of Genetics, Hebei Medical University, Shijiazhuang 050017, China
2Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
3Hebei Chemical and Pharmaceutical Vocational Technology College, Shijiazhuang 050031, China
4Pharmaceutical Preparation Section of the Third Hospital of Hebei Medical University, Shijiazhuang 050051, China

Received 13 September 2013; Revised 21 February 2014; Accepted 23 February 2014; Published 30 March 2014

Academic Editor: Patricia Valentao

Copyright © 2014 Xiaocui Duan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The study based on the chemiluminescence (CL) reaction of potassium ferricyanide and luminol in sodium hydroxide medium, enrofloxacin (ENRO) could dramatically enhance CL intensities and incorporated with matrix solid-phase dispersion (MSPD) technique (Florisil used as dispersant, dichloromethane eluted the target compounds). A simple flow injection chemiluminescence (FL-CL) method with MSPD technique for determination of ENRO in eggs was described. Under optimal conditions, the CL intensities were linearly related to ENRO concentration ranging from  g.L−1 to  g.L−1, with a correlation coefficient of 0.9989 and detection limit of  g.L−1. The relative standard deviation was 3.6% at an ENRO concentration of  g.L−1. Our testing technique can help ensure food safety, and thus, protect public health.