Construction of Differential-Methylation Subtractive Library

Table 1

Linkers, adapters, and primers used for SSH.

Group	Oligonucleotides	DNA sequence
Linker	Linker H24	AGGCAACTGTGCTATCCGAGGGAT
	Linker H12	TAATCCCTCGGA
Adapter	Ad1	CTACTACGTCGCACTGTTGGTTGGCGGGACGGCGCTCGTTAAGTC
	Ad1c	TAGACTTAACGAGC
	Ad2	TGTAGCGTGAAGACGACAGAAACCGCGTGGTGCTGAGGGCGTGAC
	Ad2c	TAGTCACGCCCT
Primer	MP1	CTACTACGTCGCACTGTTGGT
	MPN1	GCGGGACGGCGCTCGTTAAGTC
	MP2	TGTAGCGTGAAGACGACAGAA
	MPN2	CGCGTGGTGCTGAGGGCGTGAC