Construction of Differential-Methylation Subtractive Library

Table 2

DNA sequences of some fragments from the differential-methylation subtractive library.

Clone	DNA sequence
1–35	ATGATTGAGTATAACAACTTAAACTGCAACCGG*ATCTTAAAGGCGTAAG; AACTGTATCCTTGTTAGAA
2–26	GTTGTAAGGTCAGCATTTTCACTTTTCCCGGAATGAGAGATTATCAGACT; TTACATGTTGATAAGGGTAATGTTTTT
2–32	ATGATCATGGACTTATCCTACATGAGAACGCCAATGAGGATCAAGCGCGC; CTCAATGCGC**AATTAGCTGCAAGAGATGCAGCAGGGCTCGGTGTC
3–32	TAAATTAGGCATGGAACGGAGCCACTACGAAGAAGTTCCGGGGGTTACG; AAGGAAACTTCGAGTTCATATTGGTCAT
4–1	ATACAACACGAGGACTTCCCGGGAGGTGACCCATCCTAGTACTACTCCC; TCCCAAGCATGTTAACTGTGGAGTTCTGATGGGATCCGGTGCATTAGTGC; TGGTATGATTGCATCCGTTA
5–86	TTCCGAATCAATCATTGTTAACGTCACCGGAGATGATAATAAGTCGGCTT; TGTTCGGTAAGTACGATCTCGGTAAGCTCCTCGGTAGCGGCGCGTTTGCC; AAAGTCTACCAAGCGGAGGA
6–14	TTGGTGGATCCCTTAACGCGCTTCTTCTTCTTCTTTCGCGAGATAACCTA; GAAACCCTTTTCCTTTCGTTCGCTTCTTCAATCCGCCATGGGTACGGGTT; CTCGAGCTCTCTCTTTCTAGTCATTGGTCTATGTGTCTTCTTTCTCTCTCG; ATTTCTCGATTATGCGCTTACTACTTTTGTTGGTCGATCGGATCTTTTCTTC; AATTTTTGTTGAATT
7–19	TCTCTCGGGTTACAGGAAAGCTTCCACTTATCAGTCCTCCGGTGAGTTTC; AATTGCATTCATTAGTTGCCCACATAT
9–57	TAATGCCTATTGCTTTCTGATCAACTGGAATTTGAGCCAAGACATTTCCG  CGCCCAAAAGGTGTTCGATGAAATGTCTGAACCACTAATTCCAGA
10–66	TTATTAAGGCAATAGCAATGGCGCTACCGGTCTACTCGATGAACTGCTTC; TTACTTCCTACTTTAA
11–21	AGTATAAGACATAGAACCGCAACCGGATCATGAAAGCCTAAGTAGTGTTT; CCTTGTTAGAAGATAGAAAGCCAAAGACTCATACGGACTTCGGCTACA; CAATCAAAGCTATGAGAAGCAAGAAGAAGCTTTGTTAGATTTTTGTAGT; CAAATATGACTAGATGTCATGTGTATGATTGAGTATAAGAACTAGAATCGC; AACCGGATCTTAAAA

-CCGG-*: recognizing and cutting site of Hpa II.
-GCGC-**: recognizing and cutting site of Hha I.