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Journal of Chemistry
Volume 2014, Article ID 627103, 13 pages
Research Article

Optimization of Insoluble and Soluble Fibres Extraction from Agave americana L. Using Response Surface Methodology

1Université de Sfax, Laboratoire Analyses, Valorisation et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia
2Université de Sfax, Laboratoire de Chimie Industrielle, Ecole Nationale d'Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia

Received 30 May 2013; Accepted 11 October 2013; Published 5 January 2014

Academic Editor: Christophe Blecker

Copyright © 2014 Amine Bouaziz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres from Agave americana L. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W) ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affected significantly soluble fibre extraction yield and was the most important factor among nonsignificant ones influencing insoluble fibres extraction yield. Then, a Box-Behnken design was carried out to maximise fibres extraction. Selected optimal conditions were temperature: 90°C; P/W ratio: 0.1625; agitation speed: 400 rpm; and ionic strength: 1.5 g/L. These conditions yielded 93.02% and 80.46% of insoluble and soluble fibres, respectively. Concentrates showed high fibres purity and good functional properties.