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Journal of Chemistry
Volume 2014, Article ID 645687, 6 pages
Research Article

Isolation of Insecticidal Constituents from the Essential Oil of Ageratum houstonianum Mill. against Liposcelis bostrychophila Badonnel

Department of Entomology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China

Received 12 February 2014; Revised 27 May 2014; Accepted 28 May 2014; Published 15 June 2014

Academic Editor: Bhimapaka C. Raju

Copyright © 2014 Xiao Nan Lu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this research was to determine chemical composition and insecticidal activities of the essential oil of Ageratum houstonianum Mill. aerial parts against booklice, Liposcelis bostrychophila Badonnel, and to isolate any insecticidal constituents from the oil. Essential oil of A. houstonianum was obtained by hydrodistillation and analyzed by GC-MS. A total of 35 components in the essential oil were identified. The major compounds were precocene II (62.68%), precocene I (13.21%), and β-caryophyllene (7.92%). Based on bioactivity-guided fractionation, precocene II and precocene I were isolated and identified as the active constituents. The essential oil exhibited contact toxicity against L. bostrychophila with an LC50 value of 50.8  g/cm2. Precocene II (LC50 = 30.4  g/cm2) exhibited stronger acute toxicity than precocene I (LC50 = 64.0  g/cm2) against the booklice. The essential oil and the two isolated constituents also possessed strong repellent activity against L. bostrychophila. The results indicated that the essential oil and its constituent compounds have potential for development into natural insecticides or repellents for control of insects in stored grains.