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Journal of Chemistry
Volume 2014 (2014), Article ID 858106, 7 pages
Research Article

A Comparison of Second-Order Calibration Methods Applied to Excitation-Emission Matrix Fluorescence Data

Hui Chen,1,2 Zan Lin,3 Tong Wu,1 and Chao Tan1,4

1Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China
2Hospital, Yibin University, Yibin, Sichuan 644000, China
3The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
4Computational Physics Key Laboratory of Sichuan Province, Yibin University, Yibin 644007, China

Received 28 September 2014; Accepted 1 November 2014; Published 20 November 2014

Academic Editor: Tomokazu Yoshimura

Copyright © 2014 Hui Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Due to the variety of second-order data being generated by modern instruments and various mathematical algorithms being available for analysis purposes, second-order calibration is gaining widespread acceptance by analytical community. It has the so-called second-order advantage; that is, it enables concentration and spectral profiles of sample components to be extracted even in the presence of unexpected interferences. A comprehensive performance comparison of alternating trilinear decomposition (ATLD) and its two variants, that is, alternating penalty trilinear decomposition (APTLD) and self-weighted trilinear decomposition (SWATLD), was presented in this paper. The experiment was based on the simultaneous determination of three dihydroxybenzenes, that is, catechol, hydroquinone, and resorcinol, by excitation-emission matrix fluorescence (EEMF) spectroscopy. Two special measures, that is, the consistency (COS) between the resolved and actual profiles and the mean of recovery, were used for evaluation. The optimal result was obtained by the APTLD model with five components. No perceptible difference on the speed of convergence was found. It indicates that EEMF linked with the APTLD algorithm can serve as a potential tool of quantifying dihydroxybenzenes simultaneously in environmental samples.