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Journal of Chemistry
Volume 2015, Article ID 120830, 20 pages
Review Article

Chromatographic Methods in the Separation of Long-Chain Mono- and Polyunsaturated Fatty Acids

Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia in Katowice, 4 Jagiellońska, 41-200 Sosnowiec, Poland

Received 15 July 2014; Accepted 7 October 2014

Academic Editor: Hasan Uslu

Copyright © 2015 Małgorzata Dołowy and Alina Pyka. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This review presents various chromatographic systems, TLC, HPLC, GC, and also SFC, developed for identification and accurate quantification of long-chain mono- and polyunsaturated fatty acids from different samples with emphasis on selected literature which was published during last decade. Almost all the aspects such as preseparation step of fatty acids (cis and trans), stationary phase, solvent system, and detection mode are discussed.