|
| Analyte | Stationary phase | Mobile phase | Remarks | Reference |
|
| Extract of lipids coming from aquatic organisms containing PUFA | Plain silica gel plate | Hexane-diethyl ether-acetic acid 95 : 5 : 1 (v/v/v) | The plates were sprayed with sulphuric acid and next analyzed densitometrically at 600 nm | [40] |
|
| Human plasma lipids containing linoleic acid | Microscope plates coated with silica gel G | Hexane-diethyl ether-acetic acid 90 : 10 : 1 (v/v/v) | The spots were visualized by solution of 2′,7′-dichlorofluorescein in ethanol or by sulfuric acid, respectively | [61] |
|
| Deodorized fish oil containing eicosapentaenoic and docosahexaenoic acid | Argentate silica gel | Toluene-methanol 85 : 15 (v/v) | Ag-TLC method | [63] |
|
| Seafood containing saturated and unsaturated fatty acids | Silica gel coated Chromarod/Iatroscan | Hexane-diethyl ether-formic acid 99 : 1 : 0.5 (v/v/v)
Chloroform-methanol-water 5 : 4 : 1 (v/v/v) | TLC-FID method
(TLC coupled with flame ionization detector) | [73] |
|
| Milk sample containing linoleic acid | Argentate silica gel | Hexane-diethyl ether 90 : 10 (v/v) | Ag-TLC in combination with UV detection at 234 nm, the spots were visualized by solution of 2′,7′-dichlorofluorescein in methanol | [76] |
|
| Cell lipid extract containing PUFA | Silica gel plates | Chloroform-methanol-acetic acid-water
60 : 50 : 1 : 4 (v/v/v)
Heptane-diethyl ether-acetic acid 60 : 40 : 2 (v/v/v) | TLC-densitometry, after developing the plates were impregnated with cupric acetate and phosphoric acid | [77] |
|
| Fatty acids isolated from lipid-rich seeds | TLC plates (silica gel 60) | Hexane-diethyl ether-acetic acid 70 : 30 : 1 (v/v/v) | TLC with UV detection at 365 nm, ethanolic solution of 2′,7′-dichlorofluorescein was used to visualize the spots | [78] |
|
| Fatty acids separated from oleosomes of beach pea | Silica gel TLC | Hexane-diethyl ether-acetic acid 85 : 15 : 2 (v/v/v) | TLC method, iodine vapors were used to visualize the spots | [79] |
|
