Antioxidant Peptide Derived from Spirulina maxima Suppresses HIF1α-Induced Invasive Migration of HT1080 Fibrosarcoma Cells

Figure 3

S. maxima peptide attenuates CoCl₂-induced ROS level in HT1080 cells. (a) HT1080 cells (4 × 10⁴ cells/well) were incubated in the presence or absence of 100 μM CoCl₂ and 100 μM peptide for 24 h. Cellular ROS levels were assessed by DCFH-DA. All data are presented as mean ± S.D. compared with blank; compared with CoCl₂ control. (b) Representative images of HT1080 cells stained with DCF-DA. Intracellular ROS is observed with fluorescence microscopy (ZEISS, MIC00266). Bar, 10 μm. (c) Cellular ROS levels were determined by dihydroethidium (DHE) staining and flow cytometry assay. Representatives of at least 3 independent experiments are shown in the panel.

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