Antioxidant Peptide Derived from Spirulina maxima Suppresses HIF1α-Induced Invasive Migration of HT1080 Fibrosarcoma Cells

Figure 4

S. maxima peptide suppresses the HIF1α signaling pathway necessary for CoCl₂-induced invasive migration in HT1080 cells. (a) HT1080 cells were incubated with 100 μM CoCl₂ and/or 100 μM peptide for 24 h in the presence or absence of PI3K inhibitor, 10 μM LY294002 or ERK inhibitor, and 10 μM PD98059. Cell lysates were prepared, and the expression level of HIF1α and phosphorylation levels of Akt and ERK1/2 were examined by western blot analysis. Representatives of 3 independent experiments are shown. (b) Cells were prepared as in (a). Invasive migration of HT1080 cells was examined. All data are presented as mean ± S.D. compared with blank without CoCl₂ stimulation; compared with CoCl₂ control. (c) HT1080 cells were incubated with or without 100 μM CoCl₂ and 100 μM peptide for 24 h. Cell lysates were analyzed for HIF1α, β-integrin, N-cadherin, vimentin, β-catenin, and β-actin.

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