Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

Table 7

Quantification of individual HMW-GS subunits for all examined bread wheat cultivars by RP-HPLC and LoaC methods.

Subunits	LoaC		RP-HPLC
	Amount (%)^*	CV (%)	Amount (%)^*	CV (%)
5 & 2	34.0	5.62	40.0	1.06
2	25.8	40.69	37.2	7.42
5	27.5	27.16	38.9	15.75
2^*	13.8	68.72	5.3	18.71
1	13.4	14.81	6.3	44.60
7	27.6	29.45	34.0	13.37
10	21.9	30.25	10.9	38.90
8	6.4	49.85	8.8	35.21
9	17.6	26.67	13.4	22.77
12 & 9	40.4	25.20	29.2	15.87

Expressed as relative amount: percentage of individual HMW area to total HMW area.