Cartilage Tissue Engineering Using Combination of Chitosan Hydrogel and Mesenchymal Stem Cells

Figure 2

The immunohistochemistry staining results of the fabricated cartilage tissue. (a) and (b) Hematein-eosin staining. (c) and (d) Safranin O-fast green staining. (e) and (f) Type II collagen immunohistochemistry staining. (g) and (h) Aggrecan immunofluorescence imaging. Arrows in the images indicate scaffold material in the tissue. The immunohistochemistry staining results prove that the fabricated tissue has the same biochemical composition as natural cartilage tissue. Scale bar: (a), (c), (e), and (g): 100 μm; (b), (d), (f), and (h): 25 μm.

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