Journal of Chemistry

Research Article

Storage Effect on Phenols and on the Antioxidant Activity of Extracts from Anemopsis californica and Inhibition of Elastase Enzyme

Table 2

Changes in total phenols during storage of extracts from Anemopsis californica.


Part of the plant	Treatment		Storage time (days
Part of the plant	(°C)		0	30	60	90	120	180

Leaf	50	PL	27.8 ± 0.9	25.3 ± 1.2	23.1 ± 0.4	18.6 ± 0.8	15.3 ± 1.0	13.9 ± 0.2^b
	50	NP	27.8 ± 0.9	22.5 ± 0.3	20.9 ± 0.2	15.3 ± 0.1	12.8 ± 0.9	11.1 ± 0.1^a
	25	PL	27.8 ± 0.9	26.7 ± 0.9	25.9 ± 1.8	22.5 ± 0.7	19.5 ± 0.2	17.0 ± 0.6^c
	25	NP	27.8 ± 0.9	25.0 ± 0.1	23.1 ± 0.6	19.5 ± 0.8	17.2 ± 0.2	14.5 ± 0.3^b
	4	PL	27.8 ± 0.9	27.2 ± 0.9	27.1 ± 0.3	27.3 ± 0.5	21.1 ± 0.4	19.7 ± 1.1^cd
	4	NP	27.8 ± 0.9	26.7 ± 0.4	25.9 ± 0.2	21.7 ± 0.3	20.0 ± 0.6	17.8 ± 0.2^c
	−20	PL	27.8 ± 0.9	27.2 ± 0.1	27.0 ± 0.1	25.3 ± 0.3	23.6 ± 0.7	21.7 ± 0.4^d
	−20	NP	27.8 ± 0.9	27.0 ± 0.6	27.0 ± 0.3	22.2 ± 0.1	21.1 ± 0.2	19.5 ± 0.7^cd

Stem	50	PL	79.4 ± 3.5	67.5 ± 2.1	61.9 ± 2.7	56.4 ± 0.8	49.2 ± 1.1	41.3 ± 1.5^b
	50	NP	79.4 ± 3.5	64.3 ± 0.9	59.6 ± 1.6	52.4 ± 1.2	41.3 ± 1.5	34.1 ± 1.0^a
	25	PL	79.4 ± 3.5	76.2 ± 3.3	67.5 ± 0.7	63.5 ± 1.3	53.2 ± 1.4	46.8 ± 1.1^d
	25	NP	79.4 ± 3.5	75.4 ± 1.2	65.9 ± 1.7	58.8 ± 0.8	48.4 ± 0.4	44.5 ± 1.5^c
	4	PL	79.4 ± 3.5	78.6 ± 0.3	78.1 ± 1.2	68.3 ± 1.6	62.7 ± 1.1	58.0 ± 2.1^e
	4	NP	79.4 ± 3.5	77.8 ± 0.7	77.0 ± 0.9	65.1 ± 1.2	58.8 ± 1.3	54.0 ± 0.3^e
	−20	PL	79.4 ± 3.5	78.6 ± 2.3	77.8 ± 1.7	69.9 ± 0.7	64.3 ± 0.8	61.1 ± 1.1^f
	−20	NP	79.4 ± 3.5	77.8 ± 1.0	77.0 ± 0.9	67.5 ± 1.5	61.9 ± 1.2	54.8 ± 0.5^e

Rhizome	50	PL	92.3 ± 2.8	80.3 ± 1.5	71.1 ± 0.8	63.7 ± 1.3	56.3 ± 1.7	50.8 ± 0.9^b
	50	NP	92.3 ± 2.8	75.7 ± 1.9	67.4 ± 2.0	55.4 ± 0.8	48.9 ± 1.1	38.8 ± 2.2^a
	25	PL	92.3 ± 2.8	87.7 ± 1.8	78.5 ± 1.1	71.1 ± 0.5	63.7 ± 1.0	57.2 ± 2.5^d
	25	NP	92.3 ± 2.8	85.8 ± 1.1	73.8 ± 0.7	67.4 ± 1.4	58.1 ± 0.3	52.6 ± 1.2^c
	4	PL	92.3 ± 2.8	90.5 ± 1.2	90.0 ± 1.0	76.6 ± 2.1	72.9 ± 1.9	69.2 ± 2.6^ef
	4	NP	92.3 ± 2.8	89.5 ± 1.0	83.1 ± 0.6	78.5 ± 1.2	67.4 ± 2.3	60.0 ± 0.7^e
	−20	PL	92.3 ± 2.8	91.4 ± 0.9	91.0 ± 1.9	80.3 ± 1.1	75.7 ± 0.9	72.9 ± 1.6^f
	−20	NP	92.3 ± 2.8	90.5 ± 1.2	90.1 ± 1.1	79.4 ± 0.8	72.0 ± 1.9	66.5 ± 2.6^ef

Data are reported as mg gallic acid equivalents (GAE)/g of dry weight. PL: protect light using amber bottle; NP: not using amber bottle. Mean ± standard deviation (SD) of three independent experiments. Different letters in each line indicate significant difference at .