Analysis of Bovine Serum Albumin Ligands from Puerariae flos Using Ultrafiltration Combined with HPLC-MS

Table 1

The identification, retention time, UV, and MS characteristics of active compounds in Puerariae flos.

Number	Identification	(min)	Proposed ions	()	UV (nm)
1	Puerarin	12.19	[M-H]^−	415	253, 319
			[M-C4H8O4-H]^−	295
2	Daidzin	21.50	[M-H]^−	415	248, 300
			[M-glc-H]^−	253
3	3′-Methoxy daidzin	23.26	[M-H]^−	445	256, 317
			[M-glc-H]^−	283
			[M-glc-CH3-H]^−	268
4	Ononin	25.40	[M-H]^−	429	256, 320
			[M-glc-H]^−	267
			[M-glc-CH3-H]^−	252
5	Tectorigenin	29.82	[M-H]^−	299	263
			[M-CH3-H]^−	284
6	Biochanin A	38.71	[M-H]^−	283	261
			[M-CH3-H]^−	268
7	Genistein	41.30	[M-H]^−	269	268, 337
8	3′-Methoxy daidzein	41.65	[M-H]^−	283	258, 317
			[M-CH3-H]^−	268
9	Irisolidone	41.99	[M-H]^−	313	260
			[M-CH3-H]^−	298
			[M-2CH3-H]^−	283