Table of Contents Author Guidelines Submit a Manuscript
Journal of Chemistry
Volume 2015 (2015), Article ID 798304, 8 pages
http://dx.doi.org/10.1155/2015/798304
Research Article

Production and Characterization of Alkaline Protease from a High Yielding and Moderately Halophilic Strain of SD11 Marine Bacteria

1College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, China
2Hebei Province Key Laboratory of Applied Chemistry, Qinhuangdao, Hebei 066004, China
3Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia
4Flinders Centre for Nanoscale Science & Technology, School of Computer Science, Engineering and Mathematics, Flinders University, Adelaide, SA 5042, Australia

Received 16 December 2014; Revised 5 May 2015; Accepted 6 May 2015

Academic Editor: Murat Senturk

Copyright © 2015 Hongxia Cui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. B. Rao, A. M. Tanksale, M. S. Ghatge, and V. V. Deshpande, “Molecular and biotechnological aspects of microbial proteases,” Microbiology and Molecular Biology Reviews, vol. 62, no. 3, pp. 597–635, 1998. View at Google Scholar · View at Scopus
  2. O. Kirk, T. V. Borchert, and C. C. Fuglsang, “Industrial enzyme applications,” Current Opinion in Biotechnology, vol. 13, no. 4, pp. 345–351, 2002. View at Publisher · View at Google Scholar · View at Scopus
  3. V. J. Mehta, J. T. Thumar, and S. P. Singh, “Production of alkaline protease from an alkaliphilic actinomycete,” Bioresource Technology, vol. 97, no. 14, pp. 1650–1654, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. C. G. Kumar and H. Takagi, “Microbial alkaline proteases: from a bioindustrial viewpoint,” Biotechnology Advances, vol. 17, no. 7, pp. 561–594, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. R. Gupta, Q. K. Beg, and P. Lorenz, “Bacterial alkaline proteases: molecular approaches and industrial applications,” Applied Microbiology and Biotechnology, vol. 59, no. 1, pp. 15–32, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Kamath, V. M. Subrahmanyam, J. Venkata-Rao, and P. Vasantha-Raj, “Optimization of cultural conditions for protease production by a fungal species,” Indian Journal of Pharmaceutical Sciences, vol. 72, no. 2, pp. 161–166, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. C. Sandhya, A. Sumantha, G. Szakacs, and A. Pandey, “Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation,” Process Biochemistry, vol. 40, no. 8, pp. 2689–2694, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. S. K. Singh, V. R. Tripathi, R. K. Jain, S. Vikram, and S. K. Garg, “An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease,” Microbial Cell Factories, vol. 9, article 59, 7 pages, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. O.-H. Ban, S.-S. Han, and Y. N. Lee, “Identification of a potent protease-producing bacterial isolate, Bacillus amyloliquefaciens CMB01,” Annals of Microbiology, vol. 53, no. 1, pp. 95–103, 2003. View at Google Scholar · View at Scopus
  10. R. N. Z. R. A. Rahman, M. Basri, and A. B. Salleh, “Thermostable alkaline protease from Bacillus stearothermophilus F1; nutritional factors affecting protease production,” Annals of Microbiology, vol. 53, no. 2, pp. 199–210, 2003. View at Google Scholar · View at Scopus
  11. R. Gupta, K. Gupta, R. K. Saxena, and S. Khan, “Bleach-stable, alkaline protease from Bacillus sp.,” Biotechnology Letters, vol. 21, no. 2, pp. 135–138, 1999. View at Publisher · View at Google Scholar · View at Scopus
  12. K. Jellouli, A. Bougatef, L. Manni, R. Agrebi, R. Siala, and I. Younes, “Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1,” Journal of Industrial Microbiology & Biotechnology, vol. 36, no. 7, pp. 939–948, 2009. View at Google Scholar
  13. A. Ventosa, Halophilic Microorganisms, Springer, Berlin, Germany, 2004.
  14. M. A. Amoozegar, A. Z. Fatemi, H. R. Karbalaei-Heidari, and M. R. Razavi, “Production of an extracellular alkaline metalloprotease from a newly isolated, moderately halophile, Salinivibrio sp. strain AF-2004,” Microbiological Research, vol. 162, no. 4, pp. 369–377, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. C. Sánchez-Porro, E. Mellado, C. Bertoldo, G. Antranikian, and A. Ventosa, “Screening and characterization of the protease CP1 produced by the moderately halophilic bacterium Pseudoalteromonas sp. strain CP76,” Extremophiles, vol. 7, no. 3, pp. 221–228, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. C. G. Kumar, H.-S. Joo, Y.-M. Koo, S. R. Paik, and C.-S. Chang, “Thermostable alkaline protease from a novel marine haloalkalophilic Bacillus clausii isolate,” World Journal of Microbiology and Biotechnology, vol. 20, no. 4, pp. 351–357, 2004. View at Publisher · View at Google Scholar · View at Scopus
  17. L. Lama, I. Romano, V. Calandrelli, B. Nicolaus, and A. Gambacorta, “Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus,” Research in Microbiology, vol. 156, no. 4, pp. 478–484, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. A. Haddar, A. Sellami-Kamoun, N. Fakhfakh-Zouari, N. Hmidet, and M. Nasri, “Characterization of detergent stable and feather degrading serine proteases from Bacillus mojavensis A21,” Biochemical Engineering Journal, vol. 51, no. 1-2, pp. 53–63, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. R. K. Patel, M. S. Dodia, R. H. Joshi, and S. P. Singh, “Purification and characterization of alkaline protease from a newly isolated haloalkaliphilic Bacillus sp,” Process Biochemistry, vol. 41, no. 9, pp. 2002–2009, 2006. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Ç. Aksoy, A. Uzel, and E. E. H. Kocabaş, “Extracellular serine proteases produced by Thermoactinomyces strains from hot springs and soils of West Anatolia,” Annals of Microbiology, vol. 62, no. 2, pp. 483–492, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. R. K. Sarma, R. Debnath, R. Saikia, P. J. Handique, and T. C. Bora, “Phylogenetic analysis of alkaline proteinase producing fluorescent pseudomonads associated with green gram (Vigna radiata L.) rhizosphere,” Folia Microbiologica, vol. 57, no. 2, pp. 129–137, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. C. Imada, “Enzyme inhibitors of marine microbial origin with pharmaceutical importance,” Marine Biotechnology, vol. 6, no. 3, pp. 193–198, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Salwan, A. Gulati, and R. C. Kasana, “Phylogenetic diversity of alkaline protease-producing psychrotrophic bacteria from glacier and cold environments of Lahaul and Spiti, India,” Journal of Basic Microbiology, vol. 50, no. 2, pp. 150–159, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. B. K. Bajaj and P. Sharma, “An alkali-thermotolerant extracellular protease from a newly isolated Streptomyces sp. DP2,” New Biotechnology, vol. 28, no. 6, pp. 725–732, 2011. View at Google Scholar
  25. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  26. M. Kunitz, “Crystalline soybean trypsin inhibitor. II. General properties,” The Journal of General Physiology, vol. 30, no. 4, pp. 291–310, 1947. View at Google Scholar
  27. M. Cheng, S. Takenaka, S. Aoki, S. Murakami, and K. Aoki, “Purification and characterization of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4,” Journal of Bioscience and Bioengineering, vol. 107, no. 4, pp. 373–378, 2009. View at Google Scholar
  28. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar · View at Scopus
  29. W. Zhu, D. Cha, G. Cheng, Q. Peng, and P. Shen, “Purification and characterization of a thermostable protease from a newly isolated Geobacillus sp. YMTC 1049,” Enzyme and Microbial Technology, vol. 40, no. 6, pp. 1592–1597, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. L. E. Visôtto, M. G. A. Oliveira, A. O. B. Ribon, T. R. Mares-Guia, and R. N. C. Guedes, “Characterization and identification of proteolytic bacteria from the gut of the Velvetbean caterpillar (Lepidoptera: Noctuidae),” Environmental Entomology, vol. 38, no. 4, pp. 1078–1085, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. A. Anwar and M. Saleemuddin, “Alkaline proteases: a review,” Bioresource Technology, vol. 64, no. 3, pp. 175–183, 1998. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Li, B. F. He, Z. Z. Bai, and P. K. Ouyang, “A novel organic solvent-stable alkaline protease from organic solvent-tolerant Bacillus licheniformis YP1A,” Journal of Molecular Catalysis B: Enzymatic, vol. 56, no. 2-3, pp. 85–88, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. N. E. Hadj-Ali, R. Agrebi, B. Ghorbel-Frikha, A. Sellami-Kamoun, S. Kanoun, and M. Nasri, “Biochemical and molecular characterization of a detergent stable alkaline serine-protease from a newly isolated Bacillus licheniformis NH1,” Enzyme and Microbial Technology, vol. 40, no. 4, pp. 515–523, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. K. Cheng, F.-P. Lu, M. Li, L.-L. Liu, and X.-M. Liang, “Purification and biochemical characterization of a serine alkaline protease TC4 from a new isolated Bacillus alcalophilus TCCC11004 in detergent formulations,” African Journal of Biotechnology, vol. 9, no. 31, pp. 4942–4953, 2010. View at Google Scholar · View at Scopus
  35. Q. K. Beg and R. Gupta, “Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis,” Enzyme and Microbial Technology, vol. 32, no. 2, pp. 294–304, 2003. View at Publisher · View at Google Scholar · View at Scopus
  36. Y. C. Tsai, R. Y. Juang, S. F. Lin, S. W. Chen, M. Yamasaki, and G. Tamura, “Production and further characterization of an alkaline elastase produced by alkalophilic Bacillus strain Ya-B,” Applied and Environmental Microbiology, vol. 54, pp. 3156–3161, 1988. View at Google Scholar
  37. B. Jaouadi, S. Ellouz-Chaabouni, M. Rhimi, and S. Bejar, “Biochemical and molecular characterization of a detergent-stable serine alkaline protease from Bacillus pumilus CBS with high catalytic efficiency,” Biochimie, vol. 90, pp. 1291–1305, 2008. View at Google Scholar
  38. B. Ghorbel-Frikha, A. Sellami-Kamoun, N. Fakhfakh, A. Haddar, L. Manni, and M. Nasri, “Production and purification of a calcium-dependent protease from Bacillus cereus BG1,” Journal of Industrial Microbiology and Biotechnology, vol. 32, no. 5, pp. 186–194, 2005. View at Publisher · View at Google Scholar · View at Scopus
  39. C. G. Kumar, “Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus,” Letters in Applied Microbiology, vol. 34, no. 1, pp. 13–17, 2002. View at Publisher · View at Google Scholar · View at Scopus
  40. M. N. Gupta and I. Roy, “Enzymes in organic media: forms, functions and applications,” European Journal of Biochemistry, vol. 271, no. 13, pp. 2575–2583, 2004. View at Publisher · View at Google Scholar · View at Scopus
  41. K. Jellouli, O. Ghorbel-Bellaaj, H. B. Ayed, L. Manni, R. Agrebi, and M. Nasri, “Alkaline-protease from Bacillus licheniformis MP1: purification, characterization and potential application as a detergent additive and for shrimp waste deproteinization,” Process Biochemistry, vol. 46, no. 6, pp. 1248–1256, 2011. View at Publisher · View at Google Scholar · View at Scopus
  42. D. Kumar and T. C. Bhalla, “Microbial proteases in peptide synthesis: approaches and applications,” Applied Microbiology and Biotechnology, vol. 68, no. 6, pp. 726–736, 2005. View at Publisher · View at Google Scholar · View at Scopus