Production and Characterization of Alkaline Protease from a High Yielding and Moderately Halophilic Strain of SD11 Marine Bacteria

Table 4

Influence of crude alkaline protease activity by organic solvents and surfactant.

Additives	None	SDS	H2O2	Methanol	Isopropanol	Acetone
Concentration	—	5 mM	1% (v/v)	25% (v/v)	25% (v/v)	25% (v/v)
Relative activity (%)	100	95 ± 1.3	87 ± 1.1	138 ± 1.1	101 ± 1.6	141 ± 2.1

The enzyme activity was tested under normal conditions after crude enzyme was incubated in advance with various additives at 40°C and pH 10.0 for 20 min. The enzyme activity without incubating was regarded as 100%. The protease activities indicate the mean of three experiments.