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Journal of Chemistry
Volume 2015 (2015), Article ID 804658, 12 pages
Research Article

Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics

1Laboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, Tunisia
2Biology Department, College of Sciences, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia
3Unit of Research “Nitrogenized Nutrition and Metabolism and Stress Proteins” UR 99/09-20, Department of Biology, Faculty of Science, University of Tunis, 2092 Tunis, Tunisia

Received 15 December 2014; Revised 15 February 2015; Accepted 16 February 2015

Academic Editor: Augusto C. Tome

Copyright © 2015 Kamel Msaada et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability of A. absinthium L. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant. A. absinthium L. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.