Preparation and Antibacterial Properties of Substituted 1,2,4-Triazoles

Ledeţi, Ionuţ; Bercean, Vasile; Alexa, Anda; Şoica, Codruţa; Şuta, Lenuţa-Maria; Dehelean, Cristina; Trandafirescu, Cristina; Muntean, Delia; Licker, Monica; Fuliaş, Adriana

doi:https://doi.org/10.1155/2015/879343

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Research Article | Open Access

Volume 2015 | Article ID 879343 | https://doi.org/10.1155/2015/879343

Preparation and Antibacterial Properties of Substituted 1,2,4-Triazoles

Ionuţ Ledeţi,¹Vasile Bercean,²Anda Alexa,³Codruţa Şoica,¹Lenuţa-Maria Şuta,¹Cristina Dehelean,¹Cristina Trandafirescu,¹Delia Muntean,³Monica Licker,³and Adriana Fuliaş¹

Academic Editor: Teodorico C. Ramalho

Received14 Jan 2015

Revised22 Feb 2015

Accepted22 Feb 2015

Published18 Mar 2015

Abstract

Background. Both 1,2,3- and 1,2,4-triazoles are nowadays incorporated in numerous antibacterial pharmaceutical formulations. Aim. Our study aimed to prepare three substituted 1,2,4-triazoles and to evaluate their antibacterial properties. Materials and Methods. One disubstituted and two trisubstituted 1,2,4-triazoles were prepared and characterised by physical and spectroscopic properties (melting point, FTIR, NMR, and GC-MS). The antibacterial properties were studied against three bacterial strains: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 27853), by the agar disk diffusion method and the dilution method with MIC (minimal inhibitory concentration) determination. Results. The spectroscopic characterization of compounds and the working protocol for the synthesis of the triazolic derivatives are described. The compounds were obtained with 15–43% yields and with high purities, confirmed by the NMR analysis. The evaluation of biological activities showed that the compounds act as antibacterial agents against Staphylococcus aureus (ATCC 25923), while being inactive against Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). Conclusions. Our results indicate that compounds containing 1,2,4-triazolic moiety have great potential in developing a wide variety of new antibacterial formulations.

1. Introduction

Triazolic nucleus is nowadays considered an important moiety in the design and synthesis of bioactive compounds that are associated with numerous biological activities [1] such as antibacterial, antifungal [2], anti-inflammatory [3], anticonvulsant [4], anti-HIV [5], antineoplastic, and antiproliferative [6–13]. Additionally, there are review studies that indicate the fact that 1,2,4-triazoles occupy a distinctive place in the field of medicinal and pharmaceutical chemistry [14, 15], as well as in industry [16]. Also, synthesis and complete characterization by both spectroscopic and thermal techniques were reported in literature for numerous derivatives bearing 1,2,4-triazole moieties [17–20].

According to this, we set our goal in the synthesis, characterization (physical and spectroscopic properties, melting point, FTIR, NMR, and GC-MS), and evaluation of biological activity of three substitutes triazoles (1–3) against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 27853).

2. Materials and Methods

2.1. Chemistry

The reagents were commercial products of analytical purity (Chimopar, Merck, Fluka) and used as received. Melting points were determined on a Böetius PHMK (Veb Analytik, Dresden, Germany) instrument (the values are uncorrected), and thin-layer chromatography (TLC) was carried out on silica gel-coated plates 60 F254 Merck using hexane : ethyl acetate 1 : 1 (v/v) as eluant.

FTIR spectra were recorded in KBr pellet using a Specac Pellet Press (Specac Ltd., Kent, UK) on a Jasco FT/IR-410 spectrophotometer (Jasco Analytical Instruments, Easton, USA) using the following abbreviation for the aspect/intensities of the bands: br: broad; s: strong; m: medium; w: weak.

¹H-NMR and ¹³C-NMR spectra were recorded on a Bruker Avance AC200 spectrometer (Bruker Biospin International, Ag, Aegeristrasse, Switzerland) in DMSO-, using TMS as reference; chemical shifts are reported in ppm and the coupling constants in Hz. The multiplicity of the signal and the aspect of the band are abbreviated as follows: br: broad; s: singlet; d: doublet; m: multiplet.

Mass spectra GC-MS were obtained using an Agilent G1701DA apparatus (Agilent Technologies, Inc., Santa Clara, USA) using methanol as carrier solvent.

2.2. In Vitro Antibacterial Activity

The compounds were screened for their antimicrobial activity against three bacterial strains: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 27853), by the agar disk diffusion method and the dilution method with MIC (minimal inhibitory concentration) determination.

2.3. Experimental Protocols for the Synthesis of Bioactive Compounds (1–3)

The synthesis of 4H-3-mercapto-5-methyl-4-phenyl-1,2,4-triazole (1) was carried out according to a described protocol [21, 22] by heating 4-phenyl-thiosemicarbazide (4) with ethyl acetate and sodium methoxide in methanol under reflux, followed by neutralization. The synthesis of 4-phenylthiosemicarbazide was however realized in a single-step reaction, by treating aniline with CS₂ and alcoholic KOH solution, sodium chloroacetate, and hydrazine hydrate under reflux (Scheme 1) [11].

Compound 1, 4H-3-mercapto-5-methyl-4-phenyl-1,2,4-triazole, was obtained as a white powder (yield 21%), TLC one spot, m.p. 214–215°C. FTIR (KBr, cm⁻¹): 3301m; 3158m; 3097m; 3054m; 2908m; 1579m; 1498s; 1326s; 1288m; 1018w; 771w; 692m; ¹H-NMR (DMSO-, 200 MHz):13.50 (br.s, 1H, -SH); 7.30–7.65 (m, 5H, -C₆H₅); 2.08 (s, 3H, -CH₃).

1H-5-Mercapto-3-phenyl-1,2,4-triazole (2) was obtained by benzoylation of thiosemicarbazide with benzoyl chlorides, followed by cyclisation of crude 1-benzoyl-thiosemicarbazide (5) with NaOH in aqueous-alcoholic medium, using a method previously used in our research group (Scheme 2) [23].

Compound 2, 1H-5-mercapto-3-phenyl-1,2,4-triazole, was obtained as a white powder (yield 43%), TLC one spot, m.p. 242–247°C (lit. 254–256°C [24]). FTIR (KBr, cm⁻¹): 3450m; 3421m; 1630m; 1610m; 1563m; 1289m; 1080w; 776w; ¹H-NMR (DMSO-, 200 MHz): 13.87 (s, 1H, SH); 13.70 (s, 1H, NH); 7.47–7.95 (m, 5H, -Ph); ¹³C-RMN (DMSO-, 100 MHz): 172.20 (C-S); 155.41 (C=N); 135.84 (1′-C); 134.33 (4′-C); 130.86 (3′-C, 5′-C); 130.65 (2′-C, 6′-C).

4H-4-Amino-5-(4-pyridyl)-3-mercapto-1,2,4-triazole (3) was obtained using as starting compound, the hydrazide of isonicotinic acid (6), using a previously described method by us (Scheme 3) [25].

Compound 3, 4H-4-amino-5-(4-pyridyl)-3-mercapto-1,2,4-triazole, was obtained as a white powder (yield 15%), TLC one spot, m.p. 204–206°C. GC-MS (M/z): purity 99%; 193(M; 100%), 105(Py-C=NH⁺, 27%); FTIR (KBr, cm⁻¹): 3272s; 3162s; 1608m; 1318m; 826m; 736w; ¹H-RMN (DMSO-, 200 MHz): 14.18 (s, 1H, -NH); 8.77 (d, 2H, = 5.6 Hz, 3′H, 5′H); 8.04 (d, 2H, = 5.6 Hz, 2′H, 6′H), 5.87 (s.broad, 2H, -NH₂); ¹³C-RMN (DMSO-, 100 MHz): 167.6 (5-C); 150.07 (3′C, 5′C); 147.28 (3-C); 132.8 (1′C); 121.48 (2′C, 6′C).

2.4. Disk Diffusion Method

The antimicrobial activity of the compounds was evaluated according to the guidelines of the National Committee for Clinical Laboratory Standards (NCCLS, 1997) using agar disk diffusion method [26].

NCCLS recommends a bacterial suspension with a density equal to a 0.5 McFarland (which gives a final bacterial concentration of 1-210⁸ CFU/mL). The Mueller-Hinton agar plates were inoculated with bacterial suspension using a sterile cotton swab.

A series of solutions of the tested compounds were prepared in DMSO, with a concentration of 0.01%, 0.1%, 0.5%, and 1%, respectively. Within 15 min after the plates were inoculated, sterile Whatman number 1 filter paper disks (6 mm in diameter) impregnated with the solutions in DMSO of the tested compounds (20 μL solution/disk, corresponding to 2, 20, 100, and 200 μg compound/disk, resp., for the four solutions prepared with each compound) were distributed evenly on the surface, with at least 25 mm (center to center) between them. Disks with gentamicin (10 μg), supplied by Bio-Rad, were used as positive control for the antimicrobial activity. For negative control we used a paper disk impregnated with dimethylsulfoxide (DMSO).

Plates inoculated with the bacterial suspensions were incubated at 37°C for 24 h. The inhibition zone diameters were measured in millimeters, using a ruler. For all the bacterial strains the disk diffusion tests were performed in triplicate and the average reading was taken into account.

2.5. Dilution Method

The MIC values were determined only for the active compounds, with a zone of inhibition >10 mm. The MIC values were evaluated in the range of 1.56–50 μg/mL.

The MIC (μg/mL) was determined by the binary microdilution method. We prepared stock solutions in DMSO of the tested compounds, at a concentration of 100 μg/mL. From these solutions, serial dilutions of the compounds (50, 25, 12.5, 6.25, 3.12, and 1.56 μg/mL) were prepared and brought, under aseptic conditions, to a final volume of 200 μL with nutrient medium. In all tubes 50 μL of bacterial suspensions were added, with a density equal to a 0.5 McFarland. All tubes were incubated at 37°C for 24 h. The MIC was recorded as the minimum concentration of the compound which inhibited the visible growth of the tested microorganisms. For a negative control 50 μL of DMSO was introduced in a tube with 50 μL of bacterial suspension and 100 μL of nutrient medium.

We performed triplicate tests and, from all the tubes used for MICs reading, cultures on Columbia agar supplemented with 5% sheep blood were performed in order to verify the results; no bacterial growth was recorded in the presence of the tested solution to which MIC was attributed.

3. Results and Discussion

The synthesis of heterocyclic derivatives (1-3) was performed according to indications mentioned in literature. The compounds were obtained with moderate yields (15–43%) but with high purity, fact confirmed by TLC analysis and evaluation of melting interval. The formation of triazolic derivatives was proven by FTIR and NMR analysis, as well by mass spectrometry for the compound (3).

The results of the antimicrobial evaluation are presented in Table 1.

The synthesized and evaluated derivatives with triazolic structure were tested for antibacterial activity against three bacterial strains by means of the disk diffusion method. The results presented in Table 1 reveal that only two compounds may act as anti-infective agents, inhibiting the growth of S. aureus ATCC 25923 at concentrations within the range of 25–40 μg/mL. It is worth mentioning that both compounds are active against Gram-positive bacteria (S. aureus) while no activity was present against Gram-negative bacteria (E. coli, P. aeruginosa); these results demonstrate a specific antimicrobial activity against Gram-positive bacterial infections. Gentamicin is a powerful broad-spectrum antibiotic, largely used in clinical practice, usually in association with -lactam antibiotics, for the treatment of a wide range of bacterial infections; in this paper, it served as reference for the antimicrobial potency of the tested compounds. Both active substances, as showed in Table 1, produced an inhibition zone with a diameter between 11 and 17 cm at an MIC of 25–40 μg/mL. The results are comparable to the ones revealed in case of gentamicin (20 mm), demonstrating the possibility of future therapeutical use of the two compounds as potential antibacterial agents.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgment

This work was supported by a grant from the University of Medicine and Pharmacy “Victor Babeş” Timişoara (Grant II-C2-TC-2014-16498-08 to Ionuţ Ledeţi).

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Copyright © 2015 Ionuţ Ledeţi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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