Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure

Figure 4

Steps of Construction of sti1 gene/pACYC177ET3dyNMT. (A) pACYC177ET3dyNMT expression vector map. (B) 237 bp ScaI–PstI fragment containing the N-terminal half of the ampicillin-resistance gene was excised out of the pACYC177ET3dyNMT vector. (C) sti1 gene purified was digested using ScaI–PstI. (D) 1615 bp ScaI–PstI fragment was ligated to the pACYC177ET3dyNMT expression vector ScaI–PstI backbone fragment to create 6319 bp construct/sti1.