Streptomyces lavendulae Protease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure

Table 2

Purification procedures of protease inhibitor from Streptomyces.

Purification step	Total protein (mg) ± SD	Total activity (units) ± SD	Specific activity (U/mg protein) ± SD	Enzyme recovery %	Purification fold
Crude enzyme	126 ± 15	543 ± 10	4.31 ± 0.1	100	1
Amm. sulfate fraction (40–80%)	10.5 ± 14	300.1 ± 17	28.58 ± 2	55.26	6.631
Sephadex G100 fraction (25–37)	1.3 ± 12	287.8 ± 19	221.38 ± 14	53.001	51.36