Journal of Chemistry / 2015 / Article / Tab 2

Research Article

Monitoring Antibiotic Residues and Corresponding Antibiotic Resistance Genes in an Agroecosystem

Table 2

Conditions for high performance liquid chromatography-tandem mass (HPLC-MS/MS) spectrometry.

Equipment LC MS/MS (TSQ Quantum Ultra, Thermo)

LC conditionColumn temp.15°C
Column flow rate300 L min−1
Injection volume20 L
Mobile phaseA: 99.9% water + 0.1% formic acid
B: 99.9% ACN + 0.1% formic acid
GradientA: 96% + B: 4% (0 min)
A: 70% + B: 30% (29 min)
A: 96% + B: 4% (30 min)

MS conditionIon sourceESI, positive
Spray voltage4500 V
Vaporizer temp.320°C
Drying gas flow10.0 L min−1
Drying gas and nebulizer gasNitrogen gas
Sheath gas pressure40 psig
Aux gas pressure20 psig