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Journal of Chemistry
Volume 2015, Article ID 976971, 10 pages
Research Article

Polyphenolic Profile of Maize Seedlings Treated with 24-Epibrassinolide

1Institute for the Development of Water Resources “Jaroslav Černi”, Jaroslava Černog 80, 11000 Belgrade, Serbia
2Innovation Centre of Faculty of Chemistry Ltd., Studentski trg 12-16, 11000 Belgrade, Serbia
3University of Belgrade, Faculty of Chemistry, P.O. Box 51, 11158 Belgrade, Serbia
4Institute for Plant Protection and Environment, Teodora Drajzera 9, 11000 Belgrade, Serbia
5Maize Research Institute “Zemun Polje”, 11000 Belgrade, Serbia

Received 17 December 2014; Revised 13 March 2015; Accepted 13 March 2015

Academic Editor: Patricia Valentao

Copyright © 2015 Hadi Waisi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting of phenolic compounds present in seedlings of two maize genotypes ZP 434 (new generation hybrid, drought tolerant) and ZP 704 (older generation hybrid, drought sensitive) treated with different concentrations of 24-epibrassinolide. This is the first report of TLC chromatographic profile of phenolics’ mixtures in maize seed extracts influenced by brassinosteroid phytohormones. Nine samples of shoot of seedlings for the whole concentration range of phytohormones (5.2 × 10−7–5.2 × 10−15 M), one sample of root of seedlings treated with 5.2 × 10−15 M 24-epibrassinolide, and the control samples of nontreated seedlings, for both genotypes, were analyzed. Phenolic profiles of root extracts indicate the absence of more polar compounds such as phenolic acids and glycosides present in shoot of seedlings. Also, hormones applied in higher concentrations have an inhibiting effect on the content of phenolics in ZP 434. Application of chemometric methods enables characterization of particular genotype of maize according to its phenolic profile.