Journal of Chemistry

Research Article

Pepsin Digested Oat Bran Proteins: Separation, Antioxidant Activity, and Identification of New Peptides

Table 1

List of peptides identified in F3, a fraction with higher superoxide anion and hydroxyl radical scavenging activities, from HPLC separation of pepsin digested oat brans proteins on a C18 column. Scaffold software was used to validate MS/MS data. Peptide identifications were accepted if they could be established at greater than 95% probability.
Peptide sequenceObserved mass (charge)Actual massOat protein ID, UniProt database
RALPVDVL441.77 (+2)881.53P12615, P14812, P27919
SPYWNINA482.73 (+2)963.44Q38781, P12615, P14812
PQYHNAPGLV548.28 (+2)1,094.55O49257, O49258, P12615, P14812
HGQNFPILNL576.81 (+2)1,151.61O49257, O49258
NSKNFPILNI580.33 (+2)1,158.64Q38779, Q38780
VYILQGRGFTG605.83 (+2)1,209.65Q38779, Q38780, P12615, P14812
VYLLQGRGFTG605.83 (+2)1,209.65O49257
VIRRVIEPQGLL464.96 (+3)1,391.86O49257, O49258, P12615, P14812
IRRVIEPQGLLL469.63 (+3)1,405.88O49257, O49258, P12615, P14812
QQVFQPQQQAQF738.86 (+2)1,475.71I4EP64, I4EP88, F2Q9W3, F4MJY2
SVIRRVIEPQGLL493.97 (+3)1,478.90O49257, P12615, P14812
PAGIVHWGYNDGDAPVVA919.44 (+2)1,836.87O49257, O49258
QAAQRIQSQKEQRGEI618.33 (+3)1,851.96O49257, O49258
KTNPNSMVSHIAGKSSIL628.67 (+3)1,882.99O49257, O49258, Q38780, P27919
IQGHARVQVVNNNGQTVF661.02 (+3)1,980.03O49257, Q38779, Q38780
IQGRARVQVVNNHGQTVF506.53 (+4)2,022.09Q38781, P12615, P14812
DVNNNANQLEPRQKEFL677.01 (+3)2,028.00O49257, O49258, P12615, P14812, Q38780
AEGQSQSQNLKDEHQRVH523.50 (+4)2,089.99P14812
YRISRQESQNLKNNRGEE556.03 (+4)2,220.10P12615, Q38781
YRISRQEARNLKNNRGEE1117.08 (+2)2,232.14Q38779, Q38780
YRISRQEARNLKNNRGQE559.04 (+4)2,232.15O49258, P27919
YRISRQEARNLKNNRGQES580.56 (+4)2,318.19O49258, P27919
YRISRQEARNLKNNRGQESG594.81 (+4)2,375.22O49258, P27919
NAYRISRQESQNLKNNRGEE802.73 (+3)2,405.18P12615, Q38781
NAYRISRQEARNLKNNRGQE605.31 (+4)2,417.23O49258, P27919
NAYRISRQEARNLKNNRGEE605.31 (+4)2,417.23Q38779, Q38780
YRISRQEARNLKNNRGQESGV619.58 (+4)2,474.28O49258, P27919
ANAYRISRQEARNLKNNRGQE623.07 (+4)2,488.26O49258, P27919
ANAYRISRQEARNLKNNRGEE623.07 (+4)2,488.26Q38779, Q38780
NAYRISRQEARNLKNNRGQES626.83 (+4)2,503.27O49258, P27919
NAYRISRQEARNLKNNRGQESG854.44 (+3)2,560.30O49258, P27919
ANAYRISRQEARNLKNNRGQES644.59 (+4)2,574.31O49258, P27919
RQNIENPKRADTYNPRAGRITH652.60 (+4)2,606.35Q38781, P14812, P12615, O49257
ANAYRISRQEARNLKNNRGQESG658.84 (+4)2,631.33O49258, P27919
ARQNIENPKRADTYNPRAGRITH536.49 (+5)2,677.39Q38781, P14812, P12615
RALPIDVL448.78 (+2)895.55Q38779, Q38780
IRRVIEPQGLL431.94 (+3)1,292.79O49257_AVESA
QQQFQPFDQAQ682.82 (+2)1,363.62P14812, O49258
LIVPQHY435.25 (+2)868.48O49258
LLLPQYH442.26 (+2)882.50O49257, O49258, P14812, P12615
LIVPQHF427.25 (+2)852.49Q38779, Q38780
HGQNFPIL463.25 (+2)924.48O49257_AVESA, O49258
SPFWNINA474.73 (+2)947.45O49257, O49258, Q38780, Q38779
Q38779: 11S globulin, Q38780: 11S globulin, Q38781: oat storage 12S globulin, O49257: 12S globulin, O49258: 12S globulin, P12615: 12S seed storage globulin 1, P14812: 12S seed storage globulin 2, P27919: avenin, I4EP88: avenin, I4EP64: avenin, F2Q9W3: avenin protein, and F4MJY2: avenin protein.