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Journal of Chemistry
Volume 2017, Article ID 1434320, 11 pages
Research Article

Synthesis, Characterization, and Evaluation of Antimicrobial Activities of Chitosan and Carboxymethyl Chitosan Schiff-Base/Silver Nanoparticles

1Photochemistry Department, National Research Centre, El Buhouth St., Dokki, Giza 12622, Egypt
2Environmental Biotechnology and Nanotechnology, Agricultural Microbiology Department, National Research Centre, El Buhouth St., Dokki, Giza 12622, Egypt
3Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Correspondence should be addressed to Ahmed M. Khalil; moc.oohay@57lilahka

Received 23 October 2016; Accepted 26 December 2016; Published 15 March 2017

Academic Editor: Francisco Javier Deive

Copyright © 2017 Ahmed M. Khalil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Schiff-bases of chitosan (CS) and carboxymethyl chitosan (CMCS)/silver nanoparticles (AgNPs) have been synthesized, characterized, and evaluated as antimicrobial agents against two Gram +ve bacteria (Bacillus cereus and Staphylococcus aureus) and two Gram −ve bacteria (Escherichia coli and Pseudomonas aeruginosa) in addition to Candida albicans as a fungus. The in situ reactions of CS and/or CMCS with some pyrazole aldehyde derivatives in acidic media containing silver nitrate to yield silver nanoparticles loaded onto CS and CMCS/Schiff-bases were carried out. Characterizations of the prepared compounds via FTIR spectroscopy, SEM, TEM, and TGA were carried out. Schiff-bases/silver nanoparticles of CS and CMCS showed higher antimicrobial activity than the blank CS and CMCS. The presence of AgNO3 (3% wt%) displayed high antibacterial efficiencies with inhibition zones in the extent of 19–39 mm. TEM analysis showed that the size of the silver nanoparticles is in the range of 4–28 nm for the prepared nanocomposites.