Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation

Table 1

The obtained retention factor, peak asymmetry, and numbers of theoretical plates of investigated columns.

	207 nm			286 nm
	Retention factor ()	Peak asymmetry	Theoretical plates (HEPT)	Retention factor ()	Peak asymmetry	Theoretical plates (HEPT)
Gold C8	0.62	2.18	0.102	0.63	2.15	0.087
Zorbax C8	2.57	1.89	0.050	2.01	1.82	0.044
Knauer C8	2.20	2.20	0.076	1.85	2.31	0.074
Supelco C18	3.43	3.22	0.193	2.86	3.50	0.194