Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation

Table 3

Validation parameters of proposed method.

Parameter	207 nm	286 nm
Linear work range [µg/ml]	5–100	5–100
Slope ± SD	1.3286 ± 0.110	0.2544 ± 0.001
Intercept ± SD	−0.3355 ± 0.277	0.0659 ± 0.012
Coefficient of correlation	0.9990	0.9993
Limit of detection (LOD) [µg/ml]	0.33	1.19
Limit of quantification (LOQ) [µg/ml]	1.09	3.97
Intra-assay precision 1 (% RSD, = 6; 10 µg/ml)	5.97	8.23
Intra-assay precision 2 (% RSD, = 6; 50 µg/ml)	5.81	1.60
Intra-assay precision 3 (% RSD, = 6; 100 µg/ml)	6.80	1.01
Interassay precision (% RSD, = 3; 10 µg/ml)	3.44	5.66
Recovery (%, )	92.1–102.2	95.6–109.5

Intra-assay precision concentrations were 10, 50, and 100 µg/ml, respectively.