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Journal of Chemistry
Volume 2017, Article ID 6969358, 7 pages
Research Article

HPLC Quantification of Cytotoxic Compounds from Aspergillus niger

Departamento de Química Orgânica e Inorgânica, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE, Brazil

Correspondence should be addressed to Mary Anne S. Lima; rb.cfu.ioqd@yram

Received 5 December 2016; Revised 14 March 2017; Accepted 19 March 2017; Published 30 April 2017

Academic Editor: Marco Radi

Copyright © 2017 Paula Karina S. Uchoa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain of Aspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96).