Research Article
Application of Food-Grade Proteolytic Enzyme for the Hydrolysis of Regenerated Silk Fibroin from Bombyx mori
Table 4
Selection of the second proteolytic enzyme to enhance the efficiency of enzymatic hydrolysis of FoodPro-treated silk fibroin.
| Enzyme treatment | Stage | Reaction condition | Residual protein (%) | Amino acids (mg/L) | Time (h) | Temperature (°C) | pH |
| Silk fibroin | — | 0 | 60 | — | 100.0 ± 1.54c | 17 ± 0.7j | 1st step: FoodPro (FP) | 1 | 3 | 60 | 8.0 | 70.6 ± 3.81b | 2,040 ± 23.7i | 2nd step | FP ⟶ Alcalase | 2 | +3 | 60 | 8.0 | 65.5 ± 5.67b | 2,611 ± 22.0d | FP ⟶ Alphalase | 2 | +3 | 60 | 7.0 | 65.6 ± 6.83b | 2,505 ± 28.7e | FP ⟶ Bromelain | 2 | +3 | 50 | 7.0 | 68.7 ± 9.73b | 2,455 ± 16.2fg | FP ⟶ Collupulin | 2 | +3 | 60 | 6.0 | 68.5 ± 8.32b | 2,227 ± 4.7h | FP ⟶ Flavourzyme | 2 | +3 | 50 | 7.0 | 55.1 ± 2.91a | 4,519 ± 42.1a | FP ⟶ Neutrase | 2 | +3 | 50 | 7.0 | 65.4 ± 8.68b | 2,453 ± 16.2fg | FP ⟶ Pomod 192 | 2 | +3 | 50 | 5.0 | 67.1 ± 7.39b | 2,689 ± 54.7c | FP ⟶ Promod 278 | 2 | +3 | 60 | 7.0 | 67.7 ± 7.61b | 2,438 ± 35.7g | FP ⟶ Promod 279 | 2 | +3 | 60 | 5.0 | 65.0 ± 7.38b | 2,746 ± 18.7b | FP ⟶ Protamex | 2 | +3 | 50 | 7.0 | 66.6 ± 6.11b | 2,453 ± 25.7f |
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+3: additional reaction time. a–j: means in the same column followed by different letters differ significantly (p < 0.05).
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