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Journal of Chemistry
Volume 2018, Article ID 2387926, 5 pages
Research Article

Kinetics Investigation on Mushroom Tyrosinase Inhibition of Proso Millet

1Department of Applied Cosmetology, Hung-Kuang University, Taichung, Taiwan
2Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
3Department of Chemical Engineering, National United University, Miaoli City, Taiwan

Correspondence should be addressed to Yung-Sheng Lin; wt.ude.uun@synil

Received 14 October 2017; Accepted 16 January 2018; Published 12 February 2018

Academic Editor: Manuela Curcio

Copyright © 2018 Wen-Ying Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Proso millet (Panicum miliaceum) is rich in nutritive components and is widely used as a human food, feed and forage for animals, and fuel. This study investigated the effect of a proso millet extract on the inhibition of tyrosinase, a key enzyme in melanogenesis. High performance liquid chromatography analysis indicated that the proso millet extract contained phenolic tyrosinase inhibitors, such as syringic acid, -coumaric acid, and ferulic acid. The extract had an IC50 for inhibition of tyrosinase activity of 14.02 mg/mL. A Lineweaver-Burk double reciprocal plot showed that the proso millet extract functioned as a mixed competitive and noncompetitive inhibitor. Proso millet has potential as a tyrosinase inhibitor that may have applications in the cosmetics industry.