(a)
(b)
(c)
(d)
Figure 2: Effect of γ-tocotrienol on cell cycle progression, relative mRNA, and protein levels of Ki67 and PCNA in MC3T3-E1 cells. (a) Cells were treated with different doses of γ-tocotrienol for 24 and 48 h. Cells were fixed by 70% ethanol and stained by propidium iodide dye. The distribution of cell cycle was determined by flow cytometry. ((b), (c)) Cells were treated with different doses of γ-tocotrienol (2, 4, 8, and 16 μmol/L) for the desired time. mRNA levels of Ki67 and PCNA were determined by real-time PCR. (d) Protein levels of PCNA were determined by Western blot. Data are expressed as mean ± SD (). , compared to the control group. M: marker; B: blank control; C: ethanol control.