Figure 5: Effects of γ-tocotrienol on the mineralization in MC3T3-E1 cells. Cells treated with different doses of γ-tocotrienol for the desired time. The mineralization in cells was determined by Alizarin Red staining. (a) Formation of mineralized nodule (100x). (b) Alizarin Red was quantified by spectrophotometer. The results are expressed as means ± SD (). , compared to the control group.