Journal of Chemistry / 2018 / Article / Tab 1

Research Article

Comprehensive Characterization of Extractable Phenolic Compounds by UPLC-PDA-ESI-QqQ of Buddleja scordioides Plants Elicited with Salicylic Acid

Table 1

Effect of different salicylic acid concentrations on growth parameter, phenylpropanoids biosynthesis, enzymatic activities, and antioxidant capacity in a period of 14 days at 25°C.

ParameterInitial time (day zero)Salicylic acid (μM) value
0101001000

Height (cm)20.87 ± 2.69a24.00 ± 5.65a22.33 ± 1.52a36.00 ± 1.73b33.00 ± 3.00b0.001
Relative growth rate in height (day−1)0.223 ± 0.02a0.104 ± 0.02b1.080 ± 0.07d0.86 ± 0.04c0.001
Number of leaves per plant21.00 ± 3.02a25.00 ± 1.41ab27.00 ± 1.00b53.66 ± 3.05c50.66 ± 1.52c0.001
Relative effect on foliar production (leaves day−1)0.285 ± 0.04a0.428 ± 0.02b2.333 ± 0.08c2.119 ± 0.12c0.0013

Relative concentration of phenylethanoids (μg equivalent to caffeic acid g−1)
Forsythoside A6.34 ± 0.08a1.05 ± 0.03b1.39 ± 0.06c3.44 ± 0.83d1.77 ± 0.08e0.001
Verbascoside6.90 ± 0.00a1.56 ± 0.69b1.45 ± 0.06b2.62 ± 0.21c3.91 ± 0.00d0.0132
Alyssonoside1.05 ± 0.09a0.29 ± 0.04b0.18 ± 0.02cNdnd0.001
Acteoside0.54 ± 0.00a0.05 ± 0.02b0.20 ± 0.00c0.50 ± 0.06a0.30 ± 0.03d0.0728
Leucoseptoside0.69 ± 0.01a0.02 ± 0.01b0.07 ± 0.00c0.19 ± 0.01d0.07 ± 0.00c0.001

Enzymatic activities
PAL (cinnamic acid produced in μmol·mg protein−1·h−1)222.65 ± 2.95a227.37 ± 1.91a239.52 ± 0.25b251.06 ± 0.55c264.22 ± 0.48d0.001
CAT (μM·mg·protein−1·min−1)11.80 ± 0.82a11.95 ± 0.08a8.18 ± 0.10b7.38 ± 0.21c7.70 ± 0.41c0.001

Antioxidant capacity
ORAC (μM·TE/g)37.51 ± 2.80a42.15 ± 5.04ba38.11 ± 0.73a42.87 ± 0.98b44.86 ± 2.02b0.001
NO (inhibition %)17.04 ± 2.92a13.27 ± 0.02b19.87 ± 1.44a30.00 ± 1.82c31.54 ± 0.19c0.001
DPPH (inhibition %)8.92 ± 2.40a6.61 ± 0.67a16.71 ± 1.21b21.97 ± 0.57c15.95 ± 0.62b0.001

Different letters in same row indicate statistical differences by Tukey test (), (), calculated against caffeic acid at 320 nm.

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