Research Article
Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions
Figure 1
(a) Absorption spectra of the TvL, CA, and CAQ; (b) absorbance as a function of time for the oxidation reaction of different CA concentrations (λ = 410 nm); (c) spectrophotometric determination of the Michaelis–Menten kinetics for CA oxidation by TvL; (d) spectrophotometric determination of the Michaelis–Menten kinetics for GA and CT oxidation by TvL. All tests were carried out at ambient temperature in acetate buffer (0.1 M, pH 4.5).
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