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Journal of Chemistry
Volume 2018, Article ID 8673476, 12 pages
Research Article

A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Correspondence should be addressed to Huisheng Zhuang; moc.621@gnauhzgnehsiuh

Received 15 August 2017; Revised 30 November 2017; Accepted 5 December 2017; Published 4 February 2018

Academic Editor: Jorge F. Fernandez-Sanchez

Copyright © 2018 Zhen Ma and Huisheng Zhuang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods. Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP. Several physiochemical factors that may influence the assay performance were optimized in the assay. Under optimal conditions, a detection limit of 0.0094 ng mL−1 was obtained and good linearity was achieved within a range of 0.03–35.40 ng mL−1. Satisfactory recovery of spiked samples (91.12–109.23%) was obtained and the coefficient of variation was acceptable. Finally, the detection results of BaP in environmental and food samples were consistent with those obtained using high-performance liquid chromatography. The proposed immunoassay is reliable and has great potential for detecting trace amounts of BaP.