Thiadiazoline- and Pyrazoline-Based Carboxamides and Carbothioamides: Synthesis and Inhibition against Nitric Oxide Synthase

Table 2

IC₅₀ values (mM) for the inhibition of nNOS activity of compounds 5a–c and iNOS activity of 6b, 6m, 6q, and 6r. Pyrazoline 2a has been included as a control [16].

	2a	5a	5b	5c	6b	6m	6q	6r
nNOS	0.53 ± 0.12	0.42 ± 0.17	0.72 ± 0.25	0.77 ± 0.63	b	b	b	b
iNOS	b	b	b	b	0.74 ± 0.55	0.13 ± 0.14	0.53 ± 0.18	0.67 ± 0.34

obtained by measuring percentage of inhibition with at least five concentrations of inhibitor. tested.