Main Variables Affecting a Chemical-Enzymatic Method to Obtain Protein and Amino Acids from Resistant Microalgae

Table 4

Optimal values of the factors to maximize the different response variables.

Term		Response variable
		P	F	P + F	[P]	P + [P]	All
Time of the alkaline reaction (ta) (min)		120.0	108.0	106.0	111.3	111.2	106.7
Time of the enzymatic reaction (te) (min)		120.0	120.0	120.0	120.0	120.0	120.0
Temperature of the enzymatic reaction (Te) (°C)		60.0	58.0	60.0	60.0	60.0	60.0
Biomass concentration (Bc) (mg·ml^−1)		50.0	150.0	102.1	150.0	104.0	121.3
Predicted response	P	82.0	54.1	68.3	54.4	67.8	63.0
	F	0.66	1.16	1.02	1.17	1.02	1.09
	[P]	8.9	15.3	14.4	16.2	14.5	15.5

P: protein yield; F: FAN yield; +: combined optimization; [P] = protein concentration in the final extract; All: combined optimization of P + F + [P].