GC-MS Analysis and Cytotoxicity Evaluation of Shammah (Smokeless Tobacco) Samples of Jazan Region of Saudi Arabia as Promoter of Cancer Cell Proliferation

Table 2

Cytotoxic activity of the eight extracts against three cell lines and one normal fibroblast (MTT 72 h IC₅₀ ± SD μg/mL).

Sample no.	IC50 ± SD (μg/mL)
	MCF7	A2780	HT29	MRC5
S1	34.43 ± 3.62	39.60 ± 0.79	43.07 ± 1.24	23.02 ± 3.09
S2	41.47 ± 0.15	70.04 ± 0.72	39.66 ± 0.48	9.55 ± 0.44
S3	54.84 ± 1.19	48.92 ± 2.42	55.39 ± 5.68	21.28 ± 1.27
S4	26.44 ± 1.70	52.75 ± 1.51	51.29 ± 1.91	15.34 ± 1.44
S5	64.52 ± 4.92	24.00 ± 1.98	57.03 ± 3.45	16.21 ± 0.18
S6	26.23 ± 1.50	17.27 ± 1.04	39.63 ± 2.24	48.17 ± 1.03
S7	25.34 ± 1.15	44.44 ± 2.10	48.54 ± 2.62	13.19 ± 1.58
S8	35.75 ± 5.15	47.62 ± 1.23	44.45 ± 2.07	54.86 ± 2.50