Research Article

Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Figure 3

Impact of the methanol concentration in the mobile phase on the tocopherol homologues selectivity and sensitivity of the SFC-DAD method. The concentration of tocopherol homologues injected in each case was equal. The chromatographic conditions of the SFC method: isocratic flow rate: 4 mL/min; column oven temperature: 25°C; room temperature: 22°C; injection volume: 1 μL; back pressure regulator: 15 MPa; with different concentrations of methanol in the mobile phase CO2 : MeOH (v/v): 99.9 : 0.1 (a), 99.7 : 0.3 (b), 99.5 : 0.5 (c), 99.3 : 0.7 (d), 99.0 : 1.0 (e), 98.0 : 2.0 (f), 97.0 : 3.0 (g), 95.0 : 5.0 (h), and the correlations between the sensitivity (mAU) of the tocopherol homologues and the content of methanol in the mobile phase (%) (i).
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