Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

Table 3

Intra- (n = 5 × 5) and interday (n = 25 × 5) recoveries, precisions, and accuracies of tocopherols extracted from apple seeds spiked at low, medium, and high levels and detected by the SFC-DAD system.

Recovery, precision, and accuracy	Levels of tocopherol standards	Tocopherols
		α-T	β-T	γ-T	δ-T
Intraday recovery (%)	Low	95	94	89	88
	Medium	98	95	97	94
	High	99	99	98	99
Interday recovery (%)	Low	96	94	91	91
	Medium	98	97	98	96
	High	99	100	99	98
Intraday precision (CV%)	Low	5.0	6.0	6.6	5.7
	Medium	2.6	2.4	2.8	2.9
	High	2.3	2.9	2.3	3.5
Interday precision (CV%)	Low	5.7	6.7	7.0	8.0
	Medium	2.7	3.2	3.3	4.0
	High	2.0	3.2	3.0	3.4
Intraday accuracy (bias%)	Low	4.6	6.5	10.9	11.8
	Medium	2.4	4.9	2.7	5.9
	High	1.2	0.8	1.7	0.6
Interday accuracy (bias%)	Low	4.4	6.0	9.3	9.4
	Medium	2.3	3.0	2.5	4.2
	High	0.7	0.4	1.3	1.6