Synthesis and Application of Nanomagnetic Immobilized Phospholipase C

Figure 1

Effect of free PLC dosage on enzyme loading and enzyme activity. The reaction conditions were as follows: reaction temperature 55°C, pH 7.0, and reaction time 5 h. The optimum enzyme dosage was 15 mL, the enzyme loading of Fe₃O₄@SiO₂@p(GMA) was 135.64 mg/g, and the enzyme activity of PLC-Fe₃O₄@SiO₂@p(GMA) was 8560.7 U/g.