Synthesis and Application of Nanomagnetic Immobilized Phospholipase C

Figure 2

FT-IR spectrum analysis and XRD spectrum analysis. The FT-IR spectroscopy of Fe₃O₄@SiO₂@p(GMA) and PLC-Fe₃O₄@SiO₂@p(GMA) had characteristic absorption peaks at 579 cm⁻¹, 1091 cm⁻¹, 1637 cm⁻¹, and 3450 cm⁻¹, respectively. The absorption peaks presented at near 1600 cm⁻¹ belonged to ester groups of phospholipase in the FT-IR spectroscopy of PLC-Fe₃O₄@SiO₂@p(GMA). The diffraction pattern of Fe₃O₄@SiO₂@p(GMA) was close to PLC-Fe₃O₄@SiO₂@p(GMA)’s that no phospholipase crystal peak was found in it, proving phospholipase well-dispersed on carrier. (a) FT-IR spectrum analysis. (b) XRD spectrum analysis.

(a)

(b)