Research Article

Oxidative Stress and Apoptosis Contributed to Nonylphenol-Induced Cell Damage in Mouse NCTC Clone 1469 Cells

Figure 1

NP induced cytotoxicity to NCTC Clone 1469 cells. (a) Time- and dose-dependent cell viability. Cultured cells were exposed to different concentrations of NP (0, 1, 10, 20, 40, 60, and 100 μM) for 12 h or 60 μM NP for different time periods (2, 4, 6, 8, 10, 12, 24, 36, 48, 60, and 72 h). After the experiment, cell viability was examined by MTT assay. (b) Cell morphology was examined by microscopy with a photography system. (c) Effect of NP on LDH, ALT, and AST leakage in the supernatant. The activities of the LDH, AST, and ALT were expressed as % of control. Results are presented as mean ± SD with triplicate measurement. , versus the control group.