A Simple RP-HPLC Method to Simultaneously Assay the Contents of Lamivudine, Tenofovir, and Nevirapine in Fixed Dose Combined Oral Antiviral Medicines

Table 3

Optimised chromatographic conditions adopted for the validation.

Condition	Description
Column	Phenomenex Synergi C18 (250 × 4.6 mm, 4 µm)
Mobile phase	Acetonitrile (10%): ammonium acetate buffer (adjusted to pH 2.8) (40%): methanol (50%)
Diluent	Acetonitrile (10%): ammonium acetate buffer (adjusted to pH 2.8) (40%): methanol (50%)
Flow rate	1 ml/min
Column temperature	25°C
Injection volume	10 µL
Wavelength	270 nm