Research Article
Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity
Table 2
Lipase activity in crude protein extracts from domestic agricultural products (fluorometric assay).
| | Classification | Items | Concentration (mg/mL) | Activity (μU/mL) | Specific activity (μU/mg protein) | | Name | Specimen |
| | Mushrooms | Shiitake mushroom (Lentinula edodes) | Pileus | 6.0080 | 78.7792 | 13.1124 | | Stalk | 4.6380 | 12.2526 | 2.6418 | | White mushroom (Agaricus bisporus) | Whole | 7.4760 | 20.9502 | 2.8023 | | Lion’s mane mushroom (Hericium erinaceus) | Whole | 4.5490 | 275.7866 | 60.6258 | | King oyster mushroom (Pleurotus eryngii) | Pileus | 5.1730 | 526.4440 | 40.8223 | | Stalk | 4.2960 | 175.3725 | 101.7676 |
| | Vegetables | Soybean sprout (Glycine max) | Cotyledon | 16.4810 | 91.2425 | 5.5362 | | Hypocotyl | 5.0780 | 48.6468 | 9.5799 | | Onion (Allium cepa) | Whole | 4.4080 | 33.1211 | 7.5139 | | Pumpkin (Cucurbita moschata) | Whole | 5.2280 | 159.3122 | 30.4729 | | Chili pepper (Capsicum annuum) | Whole | 11.0353 | 7.8445 | 0.7109 | | Broccoli (Brassica oleracea) | Crown | 5.0830 | 426.2458 | 83.8571 | | Stalk | 7.0280 | 168.1837 | 23.9305 | | Leaf | 13.0710 | 120.8350 | 9.2445 |
| | Cereals | Job’s tears (Coix lacryma-jobi) | Whole | 8.2480 | 68.7360 | 8.3337 | | Buckwheat (Fagopyrum esculentum) | Whole | 9.2220 | 206.0473 | 22.3430 | | Pigweed (Amaranthus sp.) | Whole | 4.4660 | 73.7128 | 16.5053 | | Sorghum (Sorghum bicolor) | Whole | 4.0380 | 40.7260 | 10.0857 | | Brown rice (Oryza sativa) | Whole | 5.2450 | 105.8386 | 20.1790 |
| | Nuts/seeds | Chili pepper seed (Capsicum annuum) | Whole | 5.6420 | 554.3091 | 98.2469 | | Sunflower seed (Helianthus annuus) | Whole | 5.8240 | 7.3494 | 1.2619 | | Peanut (Arachis hypogaea) | Whole | 5.4650 | 10.6436 | 1.9476 |
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