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| Меthоd | The essence of the method | Experiment time | Experimental conditions | Applied cultures | Standard |
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| Mycological test | Determination of material resistance to the effects of mold cultures | 28 days | Temperature 29 ± 2°С Humidity over 90% Illumination 200–300 lx | Mold and microscopic fungi | ISО 846 ASTM G 21-2015 State standard 9.049–91 |
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| Bacterial test | Determination of material resistance to bacteria cultures | — | Temperature 20°С Humidity over 58% Illumination 200–300 lx | Bacterial cells | ISО 846 |
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| Biodegradability of insoluble substances | Determination of the amount of O2 absorbed during aerobic degradation | 4 months | Anaerobic conditions, buffer mineral medium, solid medium | Inoculant from activated sludge or waste water | ISO 10708 OECD 301 D |
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| Free space test at 25/50°С | Determination of the amount of CO2 released during incubation of the material | 48 days | Temperature 25−50°С, Humidity 60−70%, Illumination 200–300 lx, Anaerobic environment | Mixed population of microorganisms | ASTM D5988-96 OECD 301 A |
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| Shturm method | Determination of the released CO2 in the process of material degradation | 6 months | Temperature 20−25°С, Illumination 200–300 lx, Water solution, Aerobic environment | Fungal or bacterial flora | ISO 9439, ISO 14852, DIN EN 29439, ASTM D 5209 |
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| Determination of released СО2 (aerobic test in compost) | Determination of released CO2 in the process of material degradation in compost | 6 months | Aerobic environment, Compost based on the organic fraction of municipal solid waste | Aerobic bacteria | ISO 14855, ASTM D 6400 |
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| Determination of the degree of decomposition in compost | Determination of the degree of decomposition of material in a model industrial compost | 90 days | Temperature 58 ± 2°С, Air exchange, Darkness or stray light, Industrial compost | Inoculant, thermophilic bacteria | ISO 16929, ISO 20200, BS EN 14045 |
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| Soil test | Determination of absorbed О2 or released СО2 in the process of material degradation in natural soil of the upper layer of fields | 6 months | Temperature 28 ± 2°С, Soil moisture 30 ± 5%, pH − 7.5, Soil biological activity coefficient 0.65−1.5 | Soil microorganisms | ISO 17556, DIN 53739, State standard 9.060–75 |
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