Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting

Figure 3

Differences in gel electrophoresis amplification in four samples amplified with primers to detect fragment of 343 bp in the RAS gene. Samples N° 1, N° 2, N° 3, N° 4, and N° 6 showed reduced DNA amplification in aliquot A (fresh faeces). Only sample N° 5 shows a very intensive band in aliquot A. In these samples, aliquots stored in stabilization buffer (B and C) showed higher bands intensity with best amplification C aliquots (Buffer Genefec).